Overview

Easily study the dynamics of cellular processes with SBI’s Cyto-Tracers™

Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Apoptosis Cyto-Tracer, pCT-Apoptosis-Luc (CMV), uses cyclic luciferase as a marker for apoptosis. The cyclic luciferase is expressed from the CMV promoter for strong expression in common cell types such as HeLa, HEK293, and HT1080.

• Stable lentivector-based system
• Great for creating stable reporter cell lines
• Ideal for co-localization studies
• Monitor cellular dynamics and functional studies in real time
• Monitor apoptosis in a range of cell types

Choose the Cyto-Tracer that’s right for you

How It Works

Visualizing the cell with Cyto-Tracers

SBI’s Apoptosis Cyto-Tracer uses an inactive cyclic luciferase to monitor apoptosis via caspase 3/7 activation. The N- and C-termini of luciferase are covalently joined, thus inactivating the enzyme, via a four-amino acid peptide that is a substrate for caspase 3. Active caspase 3 digests the substrate sequence in the cyclic luciferase, separating the termini and restoring activity. Thus, with the cyclic luciferase reporter, luciferase activity increases as caspase 3 activity increases.

Supporting Data

See some of our Cyto-Tracers in action

Resources

Citations

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• Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival. Carcinogenesis.2017 Jun 1; 38(6):592-603. PM ID:28402394
• Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J. Biol. Chem..2017 Feb 10; 292(6):2470-2484. PM ID:28003368
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• VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI. Thesis.;. Link:Thesis
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• Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J. Biol. Chem..2014 Nov 21; 289(47):32526-37. PM ID:25261472
• Sato, Y, et al. (2014) MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nat Commun.2014 Nov 4; 5:5266. PM ID:25366663